Molecular Cloning A Laboratory Manual

Chapter 1. Plasmids and Their Usefulness in Molecular Cloning Chapter 2. Bacteriophage lambda and Its Vectors Chapter 3. Working with Bacteriophage M13 Vectors Chapter 4. Working with High-capacity Vectors Chapter 5. Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis Chapter 6. Preparation and Analysis of Eukaryotic Genomic DNA Chapter 7. Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells Chapter 8. In vitro Amplification of DNA by the Polymerase Chain Reaction Chapter 9. Preparation of Radiolabeled DNA and RNA Probes Chapter 10.Working with Synthetic Oligonucleotide Probes Chapter 11.Preparation of cDNA Libraries and Gene Identification Chapter 12.DNA Sequencing Chapter 13.Mutagenesis Chapter 14.Screening Expression Libraries Chapter 15.Expression of Cloned Genes in Escherichia coliChapter 16.Introducing Cloned Genes into Cultured Mammalian Cells Chapter 17.Analysis of Gene Expression in Cultured Mammalian Cells Chapter 18.Protein Interaction Technologies AppendicesSambrook, Joe is the author of 'Molecular Cloning A Laboratory Manual', published 2001 under ISBN 9780879695767 and ISBN 0879695765.