Methods in Neurosciences: Cell Culture

The ability to assess the response of neural cells is an important goal of research in the neurosciences. The means to measure resonses in a chemically defined medium is a positive feature of the use of cell culture and is the major reason its use is so important in this discipline. Key Features * Convenient benchtop format * Methods presented for easy adaptation to new systems * Comprehensive protocols included for * Preparation and maintenance of primary and continuous cultures * Cell purification * Bulk isolation * Studying pulsatility, growth on immobile carriers, rotation-mediated aggregation, microcultures, Maximow assembly cultures * Assessment of differentiated and functional aspects: endocrine modulation, morphological variants, receptors, intracellular trafficking * Clonal analysis and lineage analysisConn, P. Michael is the author of 'Methods in Neurosciences: Cell Culture' with ISBN 9780121852535 and ISBN 0121852539.