Gene Transcription: A Practical Approach

Understanding the mechanisms and control of gene transcription is one of the central goals for a large proportion of molecular biologists currently involved in research. This book concentrates on RNA polymerase II transcription of eukaryotic protein-coding systems but many of the approaches and techniques described apply equally well to the study of systems involving RNA polymerases I and III. This book covers all the major current procedures and approaches in this field, including not only the analysis of transcription in vitro and in vivo but also the identification, purification and characterization of transcription factors and their interaction with specific DNA target sites. Appendices list useful reference data including details of all known transcriptional control cis-elements and trans-factors for RNA polymerase II genes, complete with a full bibliography.Hames, B. David is the author of 'Gene Transcription: A Practical Approach' with ISBN 9780199632916 and ISBN 019963291X.