4713687
9780199632152
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This volume provides detailed protocols for a wide range of procedures relating to the electron microscopy of tissues, cells, and their components with the emphasis on biomedical applications. The fundamental methods for specimen preparation, tissue fixation, embedding, and sectioning are covered in depth and are followed by techniques in freeze-substitution, immunogold labelling, auto- radiography, and enzyme histochemistry. Although the technical details in the book relate mainly to tissues and cells, many of the procedures can be adapted for isolated organelles and membrane fractions, viral particles, macromolecular assemblies, and large proteins. This is a broadly based compilation of the most important specimen preparation techniques and their applications. It will be of interest to researchers and advanced students working in almost every biomedical discipline, plus technicians in electron microscopy units.Harris, Robin is the author of 'Electron Microscopy of Tissues, Cells, and Organelles: A Practical Approach' with ISBN 9780199632152 and ISBN 0199632154.
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