Centrosomes and Spindle Pole Bodies Methods in Cell Biology

Centrosomes and Spindle Pole Bodies contains a comprehensive collection of convenient and quantitative methods for studying these and related organelles in cells from yeast to man. Chapters outlining methods for study of the role of these organelles in other cell functions including organization of the cytoskeleton and spindle assembly are provided. Written by experts in the field, this volume provides detailed methods for the structural, biochemical, and genetic analysis of these organelles from a wide variety of experimental systems. Methods for high-resolution electron microscopy tomography, light microscopy, and fluorescence localization of proteins are also included. In addition, various approaches and strategies for the identification of organelle components and experimental strategies aimed at understanding the funciton of these molecules are described. Each chapter provides sufficient detail to allow investigators to conduct the experiments outlined in their own laboratiories. This volume should prove valuable for investigators interested in pursuing the role of centrosomes and spindle pole bodies, and related microtubule-organizing centers, in cell and developmental biology and cancer. Volume 67 of METHODS IN CELL BIOLOGY Prepared under the auspices of the American Society for Cell BiologyPalazzo, Robert E. is the author of 'Centrosomes and Spindle Pole Bodies Methods in Cell Biology' with ISBN 9780125439855 and ISBN 0125439857.