Antibodies

This convenient two-volume set discusses the properties of antibodies an antigens and provides detailed protocols for production and quality control of polyclonal, murine monoclonal and human monoclonal antibodies. Volume I contains step-by-step descriptions for such antibody methods as immunoaffinity chromatography, gel immunodiffusion, immunoelectrophoresis immunostaining, haemagglutination, and haemolysis assays. Volume II provides detailed accounts of importan labeled antibody techniques using radioisotopes, flurochromes and enzymes. Specific techniques described here include assays with radiolabels, ELISA and related enzyme immunoassays, immunoperoxidase methods, immunofluorescence, fluorescence-activated cell sorting, tumor immunodetection, blood group serology, and HLA serology. These books will be invaluable for any researcher looking to make greater use of antibodies as research tools and to update their present methodology.Catty, D. is the author of 'Antibodies', published 1989 under ISBN 9780199630639 and ISBN 0199630631.